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Holding a book that seems to thrust forward out of the picture plane is St Anthony Abbot, identified by the tau- (T-) shaped walking stick he used in later life. He also wears a black habit, which his followers, the Antonine monks, later adopted. Next to him, slightly set back, is St Francis of Assisi, with his dark brown friar's habit and a bloody wound on his hand. This is one of the stigmata – the five wounds suffered by Christ on the Cross, which were miraculously visited on the saint.
Sacchi has created a simple but effective contrast between the two meditative saints. They appear to share the same physical space, lit by a bright, raking light from the left, but they seem unaware of each other. Saint Anthony is immersed in his studies; Saint Francis's hands are crossed in religious submission, and his gaze is turned heavenwards.
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