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In the portrait study, Anna Meyer is shown seated, whereas here she is kneeling. In the drawing she cannot be older than 13-15, and given that the parents were married in 1513, Holbein must have been given the commission for the Madonna panel before he left for England in 1526. On his return, he revised the picture, putting Anna's hair up and tucking most of it under her chaplet. Smaller changes were made to the outline of her face, particularly to make the girl look somewhat older.
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